Research Paper Volume 16, Issue 14 pp 11275—11288
Interference with ANXA8 inhibits the malignant progression of ovarian cancer by suppressing the activation of the Wnt/β-catenin signaling pathway via UCHL5
Figure 5. Interference with ANXA8 inhibited the proliferation and promoted the apoptosis of CAOV3 cells through UCHL5. (A) The overexpressed plasmid of UCHL5 was constructed, and its transfection efficiency was detected by RT-qPCR; (B) The overexpressed plasmid of UCHL5 was constructed, and its transfection efficiency was detected by western blotting; ***P<0.001 vs. Ov-NC. (C) CCK-8 was used to determine cell activity; (D) EDU staining was used to detect cell proliferation; (E) TUNEL staining was used to detect the level of apoptosis; (F) Western blot analysis of the expression of apoptosis-related proteins. ***P<0.001 vs. control; ##P<0.01, ###P<0.001 vs. shRNA-ANXA8+Ov-NC.