Research Paper Volume 16, Issue 14 pp 11275—11288
Interference with ANXA8 inhibits the malignant progression of ovarian cancer by suppressing the activation of the Wnt/β-catenin signaling pathway via UCHL5
Figure 2. Interference with ANXA8 inhibited the proliferation and promoted the apoptosis of CAOV3 cells. (A) ANXA8 interference plasmid was constructed, and its interference efficacy was detected by RT-qPCR; (B) The transfection efficacy of ANXA8 interference plasmid was detected using western blotting; (C) CCK-8 assay was used to detect cell activity; (D) EdU staining was used to determine cell proliferation; (E) TUNEL staining was used to detect the level of apoptosis; (F) Western blot analysis of the expression of apoptosis-related proteins. *P<0.05, ***P<0.001 vs. shRNA-NC.