Research Paper Volume 16, Issue 12 pp 10203—10215
Aging exacerbates oxidative stress and liver fibrosis in an animal model of Down Syndrome
Figure 1. DS mice are characterized by an increased oxidative stress. (A) GSH levels are decreased in Ts2Cje 12-months old mice liver. Representative scheme for GSH quantitation in Eu and Ts2Cje livers obtained from 3-, 6-, 9-, and 12-months old mice. (B) LOOH levels are increased in Ts2Cje 12-months old mice liver. Histograms representative of LOOH spectrophotometric evaluation on Eu and TS2Cje livers obtained from 3-, 6-, 9-, and 12-months old mice. (C) HMOX1 expression is downregulated in Ts2Cje 12-months old livers. Representative histogram of the real-time PCR against HMOX1 on the liver extract obtained from 3-, 6-, 9-, and 12-months old Eu and Ts2Cje mice. GAPDH was used as housekeeping gene. Histograms are representative of four different experiments (*P ≤ 0.05; ***P ≤ 0.001).