Research Paper Volume 16, Issue 12 pp 10546—10562

Figure 6. The mechanism diagram shows that NAT1 inhibits liver metastasis of colorectal cancer. Overexpression of NAT1 inhibits PI3K/AKT/mTOR signaling pathway. On the one hand, overexpression of NAT1 inhibited the occurrence and development of EMT in tumor cells. On the other hand, NAT1 weakens the glycolytic ability of colorectal cancer cells by inhibiting the expression of GLUT1 and LDHA. The decreased glycolytic ability leads to the decreased expression of VEGF in colorectal cancer cells, and the decreased expression of VEGF leads to the decrease of neovascularization and vascular permeability in liver metastases, thus reducing the occurrence of liver metastasis.