Research Paper Volume 16, Issue 12 pp 10348—10365
Identification of JUN gene and cellular microenvironment in response to PD-1 blockade treatment in lung cancer patients via single-cell RNA sequencing
Figure 5. Validation of JUN expression in the CD8 lymphocytes as an indicative biomarker of sensitivity to PD-1 blockade in lung adenocarcinoma. (A) Schematic showing the RNA-seq validation of procedures used for JUN gene (created using BioRender.com). (B) Heat map of immune cell fractions in the microenvironment of lung cancer patients (n=17, LUAD: 3 patients, LUSC: 14 patients). (C) Box and whisker plot showing the JUN expression level of poor or good response for PD-1 blockade therapy in lung cancer patients using RNA sequence (TPM; Log10; good response: n = 8; poor response: n = 9; P=0.02) with non-parametric Wilcoxon test. (D) Kaplan-Meier plotter showing the probability of JUN in lung cancer patients (Logrank test, P=0.00023).