Research Paper Volume 16, Issue 11 pp 9933—9943

Figure 3. Rg3 enhances proliferation, attenuates apoptosis, and activates the MAPK pathway through the downregulation of miR-216a-5p in HG-induced SV40 MES 13. (A) qRT-PCR analysis for the evaluation of a miR-216a-5p expression in HG-induced SV40 MES 13 after processing with Rg3. (B) HG-induced SV40 MES 13 were transfected with miR-216a-5p inhibitor, miR-216a-5p mimics or NC, and treated with Rg3. The expression change of miR-216a-5p was identified by applying qRT-PCR. (C) CCK-8 assay for the examination of cell proliferation in SV40 MES 13 treated in the same way as B. (D) TUNEL staining revealed the change of cell apoptosis (Magnification, 200×). (E) TUNEL-positive cells were quantified. (F) The changes of cle-Caspase 3, cle-Caspase 8, Bcl2, and Bax expressions were confirmed through the application of western blot. (G) Western blot analysis of p-p70s6k, p70s6k, P-MAPK, and MAPK expressions in each group. ^***P < 0.001 vs. control group; ^&&P < 0.01, ^&&&P < 0.001 vs. HG + NC group; ^#P < 0.05, ^##P < 0.01, ^###P < 0.001 vs. HG + mimics group.