Research Paper Volume 16, Issue 11 pp 9876—9898
High estrogen induces trans-differentiation of vascular smooth muscle cells to a macrophage-like phenotype resulting in aortic inflammation via inhibiting VHL/HIF1a/KLF4 axis
Figure 5. Effect of high-dose estrogen on the expression of MOVAS cell markers. (A) MOVAS cells were treated with 10 μM E2 with or without TAM. After 4 days, the contraction phenotypic markers and macrophage-like phenotypic markers of MOVAS cells were detected by qRT-PCR as well as the mRNA expression of related cellular pathways (n = 4). (B) MOVAS cells were treated with 10 μM E2 with or without kenpaullone. After 4 days, the mRNA expression levels of related genes were detected by qRT-PCR (n = 4). (C) MOVAS cells were treated with 10 μM E2 with or without VH-298 for 4 days, and the mRNA expression levels of related genes were detected by qRT-PCR (n = 4). *VS control group, *P < 0.05, **P < 0.01, indicating statistically significant data between groups; #VS. E2 group, #P < 0.05, ##P < 0.01, indicating statistically significant data between groups; ns indicates no statistical significance.