Research Paper Volume 16, Issue 11 pp 9846—9858

Figure 2. MIR181A2HG negatively regulated keratinocytes proliferation. (A) The efficiency of interference and overexpression of MIR181A2HG in HaCaT keratinocytes was detected. (B) The effects of interference or overexpression of MIR181A2HG on cell viability were assessed using CCK-8 kit. (C) HaCaT keratinocytes transfected for 48 hours were harvested and qRT-PCR was used to evaluate the effects of interference or overexpression of MIR181A2HG on KRT6 and KRT16 mRNA level. (D) Western blotting was applied to detect the effects on Cyclin A2, CDK4, Cyclin D1, KRT6 and KRT16 protein level. GAPDH served as an internal reference. (E) Flow cytometry was performed to analyze the effects on cell cycle distribution. *P<0.05, **P<0.01, ***P<0.001.