Research Paper Volume 16, Issue 11 pp 9727—9752

Figure 9. 14-3-3σ promotes the carbon ion radiosensitivity of PAAD cells in vivo. (A) The overall design of the in vivo experiments. (B–D) Macro images, tumor volume, and tumor weight of the excised tumors for each group (4 mice/group). (E, F) Representative 40× images of TUNEL immunofluorescence (IF) staining and immunohistochemical (IHC) staining of Ki67 of tumor tissue in sections in different groups at 24 h after 5 Gy carbon ion irradiation. (G, H) Respective percentage of TUNEL-positive cells in the excised tumor by quantitative analysis. (H) Respective percentage of Ki67-positive area in the excised tumor by quantitative analysis. (I, J) Representative 40× images of IHC staining of γH2AX, and RPA2 of tumor tissue in sections in different groups at 24 h after 5 Gy carbon ion irradiation. (K, L) Respective percentage of γH2AX-, RPA2-positive area in the excised tumor by quantitative analysis. Data were presented as the mean ± standard deviation (SD); *p <0.05, **p <0.01, ***p <0.001, ****p <0.0001, ns: not significant. Scale bar = 50 μm.