Research Paper Volume 16, Issue 11 pp 9727—9752

Figure 5. 14-3-3σ involves the accumulation of DSBs. (A) The representative images of the γH2AX and P53 binding protein 1 (53BP1) foci in AsPC-1 cells after 3 Gy carbon ion irradiation in different groups were analyzed by immunofluorescence (IF) staining. (B, C) Percentage of positive nuclei of γH2AX and 53BP1 in AsPC-1 cells in different groups (5 foci or more per nucleus being considered positive). (D) The representative images of the γH2AX and 53BP1 foci in MiaPaCa-2 cells after 3 Gy carbon ion irradiation in different groups were analyzed by IF staining. (E, F) Percentage of positive nuclei of γH2AX and 53BP1 in MiaPaCa-2 cells in different groups (5 foci or more per nucleus being considered positive). (G, H) Relative expression of 53BP1 and γH2AX in si14-3-3σ and NC AsPC-1 cells, 0 h, 1 h, 4 h, 8 h, and 12 h post-irradiation. (I, J) Relative expression of 53BP1 and γH2AX in 14-3-3σ-overexpression and vector MiaPaCa-2 cells, 0 h, 1 h, 4 h, 8 h, and 12 h post-irradiation. For IF, at least 100 cells per condition were analyzed. β-Actin was used as a loading control. Data were presented as the mean ± standard deviation (SD); *p <0.05, **p <0.01, ***p <0.001, ****p <0.0001, ns: not significant. Scale bar = 10 μm.