Research Paper Volume 16, Issue 11 pp 9727—9752
14-3-3σ downregulation sensitizes pancreatic cancer to carbon ions by suppressing the homologous recombination repair pathway
Figure 4. 14-3-3σ is responsive to DNA damage in PAAD cells. (A–C) The protein and mRNA levels of 14-3-3σ in AsPC-1 cells after 3 Gy carbon ion irradiation at 1 h, 4 h, 8 h, and 12 h. (D–F) The protein and mRNA levels of 14-3-3σ in PANC-1 cells after 3 Gy carbon ion irradiation at 1 h, 4 h, 8 h, and 12 h. (G–J) The protein levels of 14-3-3σ expression in AsPC-1 cells with chemotherapeutics at 1 h, 4 h, 8 h, and 12 h. β-Actin was used as a loading control. Data were presented as the mean ± standard deviation (SD) (n=3); *p <0.05, **p <0.01, ***p <0.001, ****p <0.0001, ns: not significant.