Research Paper Volume 16, Issue 11 pp 9727—9752

Figure 2. Knockdown of 14-3-3σ sensitizes PAAD cells. The level of 14-3-3σ expression in PAAD cell lines (AsPC-1, SW1990, PANC-1, MiaPaCa-2) was detected by immunoblotting (A, B) and qPCR (C). The protein and mRNA levels of 14-3-3σ in AsPC-1 cells with 14-3-3σ-specific small interfering RNA (siRNA) were detected by immunoblotting (D, E) and qPCR (F). (G, H) Colony formation assays and survival fraction curves of AsPC-1 cells after exposure to the indicated carbon ion irradiation dose (1 Gy, 2 Gy, and 3 Gy). (I) Cell proliferation of AsPC-1 cells in different groups after 3Gy carbon ion irradiation was determined by CCK-8 assay at 24 h, 48 h, and 72 h. (J, K) The proliferation of AsPC-1 cells was detected by EdU assay. Scale bar = 100 μm. (L, M) The apoptosis rate of AsPC-1 cells after 3 Gy carbon ion irradiation at 24 h in different groups. Scale bar = 10 μm. (N, O) The cell cycle distribution of AsPC-1 cells after 3 Gy carbon ion irradiation at 6 h, 12 h, and 24 h in different groups. β-Actin was used as a loading control. Data were presented as the mean ± standard deviation (SD) (n=3); *p <0.05, **p <0.01, ***p <0.001, ****p <0.0001, ns: not significant.