Research Paper Volume 16, Issue 11 pp 9680—9691
Daphnoretin inhibited SCI-induced inflammation and activation of NF-κB pathway in spinal dorsal horn
Figure 4. Daphnoretin inhibited LPS-induced NF-κB pathway activation and inflammation in BV2 cells. (A) The mRNA expression of TNF-α and IL-1β in BV2 cells were measured by PCR. (B) The protein expression of TNF-α and IL-1β in BV2 cells were measured by ELISA. (C, D) The protein expression of p-NF-κB, NF-κB p-IκBα and IκBα were examined by Western blot. n = 3 /group, *P < 0.05, ***P < 0.01, ****P < 0.0001 vs Sham group, #P < 0.05, ##P < 0.01, ####P < 0.0001 vs SCI group, the data were analyzed by one-way ANOVA with Tukey’s test.