Research Paper Volume 16, Issue 11 pp 9518—9546

Figure 8. Expression analysis and biological function assessment of NFE2L2. (A) NFE2L2 expression in normal tissues and gliomas was examined using TCGA and GTEx data. (B) Quantification of NFE2L2 expression levels from CGGA datasets. (C) Typical IHC images of NFE2L2 expression in the HPA database. (D) qRT-PCR analysis of NFE2L2 mRNA expression in human astrocytes (NHA) and glioma cell lines (H4, T98G). (E) Validation of NFE2L2-specific shRNA knockdown efficiency in T98G and H4 cell lines by qRT-PCR. (F) Assessment of glutathione levels in T98G and H4 cells after NFE2L2 knockdown. (G) Impact of NFE2L2 knockdown on the proliferation of T98G and H4 cells. (H) Colony formation in response to NFE2L2 knockdown in T98G and H4 cell lines. (I) Histograms showing the number of colonies. (J) Scratch experiments revealing the effect of NFE2L2 knockdown on the migratory capacity of T98G and H4 cell lines. (K) Histograms showing the migration rate (N=5 or N=6). All data points were presented as mean ± SD, from three to six independent experiments.