Research Paper Volume 16, Issue 11 pp 9410—9436

Figure 6. Elevated APOA5 enhances stemness traits of primary CRC cells. (A) Expression levels of stemness-related factors, LGR5 and ALDH1A1 in APOA5 overexpressed and parental primary cells, which was analyzed with Western blot assays. (B) ALDH1^high proportion of APOA5 overexpressed and parental cells were determined by flow cytometry analysis. (C) The percentage of LGR5 positive cells in APOA5 overexpressed and parental cells were analyzed with flow cytometry analysis. Data are shown as the mean +/− SEM. (D) Tumorosphere formation efficiency was compared between APOA5 overexpressed and parental cells. Results are representative of at least three independent experiments. (E) Tumorigenesis ability of HCT116-APOA5 and parental cells was analyzed with subcutaneous xenografts by gradient number of cancer cells in vivo. (F) Growth curves of tumors (1 × 10⁶ cells injected) showed increased tumor growth of HCT116/APOA5 than control cells (n = 6 each group). p-values < 0.05 are represented as a^*.