Research Paper Volume 16, Issue 7 pp 6566—6587
Transcranial direct current stimulation promotes angiogenesis and improves neurological function via the OXA-TF-AKT/ERK signaling pathway in traumatic brain injury
Figure 7. Orexin-A improved scratch wound healing and angiogenesis of TNF-α-injured HUVEC cells. (A) The image shows the effect of Orexin-A (TO1: 0.1 μM, TO2: 0.5 μM and TO3: 1 μM) on wound healing of TNF-α (10 ng/ml) injured cells. (B) Quantification of Orexin-A on wound closure (n = 3). (C) Representative images of the study effects of TNF-α (10 ng/ml) and the three concentrations (TO1: 0.1, TO2: 0.5 and TO3: 1 μM) of Orexin-A on tube formation. (D) Quantification of the effects of Orexin-A on the number of capillary-like tubes in TNF-α-treated cells (n = 3). Results are expressed as means ± standard deviation, the statistical significance of differences was evaluated by One-way ANOVA, *denotes the comparison between the Sham group and the TBI group, #represents the comparison between the TBI and the Sham-tDCS group, &signifies the comparison between the tDCS group and the Sham-tDCS group, and ¥indicates the comparison between the tDCS group and the tDCS+SB334867 group. P < 0.05 indicates statistical significance.