Research Paper Volume 16, Issue 7 pp 6521—6536

Figure 6. GO inhibits the NF-κB signaling and NLRP3 inflammasome-mediated inflammatory pyroptosis in LPS-induced ALI via the H₂S-generating pathway. (A–D) ELISA of the levels of inflammatory cytokines, including TNF-α, IL-6, IL-1β, and IL-18, in lungs. (E–H) ELISA of the levels of secreted inflammatory cytokines, including TNF-α, IL-6, IL-1β, and IL-18, in BALF. (I) Western blotting to detect the protein expression levels of p-p65, p65, NLRP3, ASC, c-caspase 1, and apoptotic markers in lungs. (J) The grayscale values of the bands in the image were normalized to β-actin and analyzed using ImageJ software. Data are expressed as the means ± SD. Student’s t-test, ^*p < 0.05, ^**p < 0.01, and ^***p < 0.001. Abbreviation: ns: no significant difference.