Research Paper Volume 16, Issue 7 pp 6521—6536

Figure 5. GO alleviated LPS-induced ALI via the H₂S generating pathway. (A) Western blotting to detect the protein expression levels of three H₂S-producing enzymes, namely CBS, CSE, and 3MST, in lung tissue. (B) The grayscale values of the bands in image A were normalized to β-actin and analyzed using ImageJ software. (C) Detection of H₂S levels in lung tissues. (D) Flow diagrams of mice experiments. IAM is an effective inhibitor of the conversion of DATS to H₂S in vivo. For IAM delivery, mice were administered 3% IAM in their drinking water 30 min after LPS instillation. AOAA is a CBS inhibitor and PAG is a CSE inhibitor. For AOAA and PAG delivery, mice were intraperitoneally injected with AOAA (50 mg/kg) and PAG (100 mg/kg), respectively, 30 min after LPS instillation. (E) Lung tissue sections stained with hematoxylin and eosin (H&E staining, scale bar: 50 μm). (F) Severity of lung damage was scored on a scale of 0–10 according to inflammatory cell infiltration, alveolar septal edema and thickening, hyaline membrane formation, and pulmonary hemorrhage (n = 8 per group). (G) The total number of cells exuded in BALF. Data are expressed as the mean ± SD. Student’s t-test, ^*p < 0.05, ^**p < 0.01, and defined as ^***p < 0.001. Abbreviation: ns: no significant difference.