Research Paper Volume 16, Issue 7 pp 6521—6536

Figure 4. GO blocks the expression of pyroptosis-related proteins in LPS-induced ALI. (A) mRNA expression levels of the active forms of the pyroptosis-related proteins GSDMD and GSDME in lung tissue were analyzed via real-time PCR. (B) Protein expression levels of the active forms of the pyroptosis-related proteins GSDMD and GSDME in lung tissue were analyzed via western blotting. (C) A histogram of the grayscale values of the protein bands calculated using ImageJ software (standardized to β-actin). (D) The pyroptosis rate of isolated primary alveolar epithelial cells was analyzed using flow cytometry and FLICA caspase-1 and PI double staining. (E) The incident rate of pyroptosis was calculated for each group. Experiments were performed in triplicate, and data are expressed as the mean ± standard deviation (SD). Student’s t-test, ^*p < 0.05, ^**p < 0.01, and ^***p < 0.001. Abbreviation: ns: no significant difference.