Research Paper Volume 16, Issue 7 pp 6521—6536

Figure 2. GO blocks lung inflammation during LPS-induced ALI. (A) Real-time PCR analysis showed that GO prevented the increase in the mRNA expression levels of inflammatory cytokines, including TNF-α, IL-6, IL-1β, and IL-18, in lung tissues. (B) Western blotting revealed that GO blocked the increase in the protein expression levels of inflammatory cytokines, including TNF-α, IL-6, IL-1β, and IL-18, in lung tissues. (C) Statistical chart of density analysis using ImageJ. (D–G) ELISA analysis revealed that GO inhibited the secretion of inflammation cytokines, including TNF-α, IL-6, IL-1β, and IL-18, in BALF. Experiments were performed in triplicate, and data are expressed as the mean ± standard deviation (SD). Student’s t-test, ^*p < 0.05, ^**p < 0.01, and ^***p < 0.001. Abbreviation: ns: no significant difference.