Research Paper Volume 16, Issue 7 pp 6510—6520
has-miR-134-5p inhibits the proliferation and migration of glioma cells by regulating the BDNF/ERK signaling pathway
Figure 3. BDNF is a downstream target gene of hsa-miR-134-5p, which exerts its inhibitory effect on the cell cycle by suppressing the expression of proteins involved in cell cycle regulation and matrix proteases. (A) TargetScan to anticipate that BDNF would be the downstream gene of hsa-miR-134-5p. (B) Western blot shows the expression levels of cyclin D1, cyclin A2, and MMP2/9 were significantly reduced in U251 and U87 cells transfected with hsa-miR-134-5p mimics compared to those transfected with negative control. (C) Flow cytometry analysis revealed an increased proportion of G0/S phase cells in the hsa-miR-134-5p mimic group compared to cells transfected with NC. Conversely, a decreased proportion of G0/S phase cells was observed in the hsa-miR-134-5p inhibitor group.