Research Paper Volume 16, Issue 7 pp 6334—6347
Lactobacillus plantarum 45 activates SHP2 through inhibition of oxidative stress to regulate osteoblast and osteoclast differentiation
Figure 1. Detection of oxidative stress level and protein expressions of NOX4, P22 and P47 in macrophages via immunofluorescence and Western blotting, respectively, and detection of MDA and SOD concentrations via ELISA. (A) Detection of oxidative stress level of macrophages in each group via immunofluorescence and relative fluorescence intensity of ROS in LPS group and LPS + LP45 group. (B) Detection of protein expressions of NOX4, P22 and P47 in the four groups of macrophages via Western blotting. (C) Protein expressions of NOX4, P22 and P47 in macrophages. (D) Detection of MDA and SOD concentrations via ELISA. **p < 0.05, Abbreviation: ns: no statistically significant difference.