Research Paper Volume 16, Issue 7 pp 6147—6162

Figure 8. Effect of Poria cocos polysaccharides (PCPs) on the mRNA expression of Nrf2, ferroptosis, and inflammatory factors in BLR3A cells. (A) NF-κβ. (B) A comparison of the MyD88 results. (C) The comparison of Nrf2 results. (D) The comparison of glutathione results. (E) A comparison of the FTH1 results. (F) A comparison of the interleukin (IL-1β) results. (G) A comparison of the IL-6 results. (H) A comparison of the GPX4 results. (I) A comparison of the results of HO-1. Compared with the control group, after intervention with alcohol and ML385, the mRNA levels of Nrf2, GSH, FTH1, HO-1 and GPX4 in BLR3A cells were significantly reduced (P<0.01). After intervention with PCP and Fer-1, compared with the ALD group and the ALD+ML385 group, the mRNA expression of Nrf2, GSH, FTH1, HO-1, and GPX4 in BLR3A cells was significantly increased (P<0.05). Compared with the control group, the levels of mRNA of IL-1β, IL-6, NF-κβ and MyD88 after intervention with alcohol and ML385 were significantly increased (P<0.01). After intervention with PCP and Fer-1, compared with the ALD group and the ALD+ML385 group, the mRNA expression of IL-1β, IL-6, NF-κβ and MyD88 in BLR3A cells was significantly reduced (P < 0.01). The reported values are presented as mean ± SD, n = 3. ^*P<0.05, ^***P<0.01, compared with the control group; ^#P<0.05, ^###P<0.01, compared with the ALD group; ^xP<0.05, ^xxxP<0.01, compared with the ALD+ML385 group.