Research Paper Volume 16, Issue 7 pp 6147—6162

Figure 7. Effect of Poria cocos polysaccharides (PCPs) on intracellular inflammatory factors, Nrf2, and Fer. After treatment with 150 mM alcohol for 24 hours, the alcohol liver injury cell model was established, and PCP (100 μg/mL) or ferroptosis inhibitor Fer-1 (100 μg/mL) intervention was carried out for 24 hours. After fluorescent staining, inflammatory factors, Nrf2, and Fer expression levels were observed under a fluorescent microscope. The rats were treated with 150 mM alcohol for 24 hours, followed by intervention with the Nrf2 inhibitor ML385 (5 μg/mL) for 2 hours and the addition of PCP (100 μg/mL). The expression of inflammatory factors, Nrf2, and Fer was observed under a fluorescence microscope after fluorescent staining. The reported values are presented as mean ± SD, n =3. ^*P<0.05, ^***P<0.01, compared with the control group; ^#P<0.05, ^###P<0.01, compared with the ALD group; ^xP<0.05, ^xxxP<0.01, compared with the ALD+ML385 group.