Research Paper Volume 16, Issue 7 pp 6147—6162

Figure 6. Effects of Poria cocos polysaccharides (PCPs) on the mRNA expression of Nrf2, ferroptosis, and other indicators in the liver tissue of alcoholic liver disease (ALD) rats. (A) The effect of PCP on the inflammatory signaling pathway in the liver tissue of ALD rats. Compared with the control group, interleukin (IL)-1β, IL-6, P65, P-P65, MyD88 were significantly increased in the liver tissue of rats subjected to intervention with alcohol and ML385 (P < 0.05); after the intervention, compared with the ALD model group and the ML385 group, the expression of IL-1β, IL-6, P65, P-P65, and MyD88 in the rat liver tissue was significantly reduced (P<0.05). After intervention with PCP and Fer-1, the expression of IL-1β, IL-6, P65, P-P65, and MyD88 in the rat liver tissue was significantly decreased (P<0.05). (B) The effects of PCP on Nrf2 and ferroptosis-related signaling pathways in the liver tissue of ALD rats. Compared with the control group, Nrf2, GSH, FTH1, HO-1, GPX4 were significantly reduced in the liver tissue of rats after alcohol and ML385 intervention (P<0.05); after PCP (100 mg/kg) intervention, compared with the model group and the ALD+ML385 group, the expression of Nrf2, GSH, HO-1, GPX4 in the rat liver tissue was significantly increased (P<0.05). After intervention with PCP and Fer-1, the expression of Nrf2, GSH, HO-1, and GPX4 in the liver tissue of rats was significantly increased (P<0.05). Although the expression of the FTH1 protein in the ALD+PCP group and Fer-1 showed a downward trend, no significant difference was observed when compared with the ALD group (P<0.05). The reported values are presented as mean ± SD, n = 3. ^*P<0.05, ^***P<0.01, compared with the control group; ^#P<0.05, ^###P<0.01, compared with the ALD group; ^xP<0.05, ^xxxP<0.01, compared with the ALD+ML385 group.