Research Paper Volume 16, Issue 7 pp 5967—5986
Identification and validation of DHCR7 as a diagnostic biomarker involved in the proliferation and mitochondrial function of breast cancer
Figure 8. DHCR7 knockdown inhibited proliferation and induced apoptosis of MDA-MB-231 cells. (A) Correlation between DHCR7 expression and 11 genes related to breast cancer proliferation. (B) GSEA analysis of DHCR7 expression in BC. (C, D) qRT-PCR and western blot determined the efficiency of DHCR7 knockdown in MDA-MB-231 cells. (E) MTT assay investigated the MDA-MB-231 cells viability with DHCR7 knockdown. (F) The effect of DHCR7 knockdown on colony formation of MDA-MB-231 cells. (G) Western blot detected the expression of CDK6, caspase9, and Bcl2. (H) Flow cytometry assay tested the cell cycle of MDA-MB-231 cells with DHCR7 siRNA. (I) DHCR7 knockdown remarkably induced apoptosis of MDA-MB-231 cells. Abbreviation: NC: negative control. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.