Research Paper Volume 16, Issue 7 pp 5929—5948

Figure 5. EIF3D regulates gene expression in FaDu cells. (A) Boxplot illustrating the expression levels of EIF3D in RNA-seq data. (B) PCA based on FPKM values of all detected genes. The ellipse for each group represents the confidence ellipse. (C) Volcano plot displaying all differentially expressed genes (DEGs) between treatment and control samples with DEseq2. P-value < 0.01 and FC (fold change) ≥ 1.5 or ≤ 0.67. (D) Hierarchical clustering heatmap depicting expression levels of all DEGs. (E) Bubble diagram presenting the most enriched GO biological process results of the up-regulated and down-regulated DEGs. (F) Boxplot showing the expression pattern and statistical differences of immune-related DEGs. Error bars represent mean ± SEM. ^*P-value < 0.05, ^**P-value < 0.01, ^***P-value < 0.001.