Research Paper Volume 16, Issue 7 pp 5916—5928
Downregulating miRNA-199a-5p exacerbates fluorouracil-induced cardiotoxicity by activating the ATF6 signaling pathway
Figure 3. ATF6 is activated in 5-FU treated myocardial tissues. (A) The potential target of miRNA-199a-5p were explored by online tools and ATF6 was confirmed has potential binding sites with miRNA-199a-5p; (B) the expression of ATF6, GRP78, CHOP, PERK protein increased in 5-FU treated cardiomyocytes tissues; (C) the qRT-PCR analysis of ATF6 expression in cardiomyocytes; (D) the correlation of ATF6 and miRNA-199a-5p by RT-PCR analysis. ATF6 is negatively correlated with miRNA-199a-5p in 5-Fu treated myocardial tissues.