Research Paper Volume 16, Issue 6 pp 5000—5026

Figure 6. Effect of GNL on D-gal-induced cognitive impairment mice of RAGE and BACE-1 proteins in the hippocampus. (A) A Western blot analysis of the RAGE and BACE-1 protein levels. Three independent experiments are shown here. SDS-PAGE resolved the protein from each sample, and Western blots were done. The internal load controllers were β-actin. Densitometry analysis calculated changes in protein bands as 1.0-fold, as shown below the gel. (B) 4-month-old young control and 16-month-old mouse hippocampus tissue were analyzed for RAGE and BACE-1. The internal load controllers were β-actin. Densitometry analysis calculated changes in protein bands as 1.0-fold. Group I: Control; Group II: D-gal alone (150 mg/wt); Group III: D-gal (150 mg/wt) with GNL (40 mg/wt); and Group IV: GNL alone (40 mg/wt). Group V: 4-month-old young animals; Group VI: 16-month-old Values are expressed as the mean ± SD (n = 6). ^*P < 0.05 vs. control group; ^#P < 0.05 vs. D-gal alone-treated mice. ^@P < 0.05 young vs. old mice.