Research Paper Volume 16, Issue 6 pp 5000—5026

Figure 5. Effect of GNL on D-gal-induced cognitive impairment mice of Nrf2, HO-1, and NQO-1 proteins in the hippocampus. (A) The total Nrf2, HO-1, and NQO-1 protein levels were analyzed by Western blot. Three independent experiments are shown here. SDS-PAGE resolved the protein from each sample, and Western blots were done. The internal load controllers were β-actin. Densitometry analysis calculated changes in protein bands as 1.0-fold, as shown below the gel. (B) 4-month-old young control and 16-month-old mice hippocampus tissue were analyzed for total Nrf2, HO-1, and NQO-1. The internal load controllers were β-actin. Densitometry analysis calculated changes in protein bands as 1.0-fold, as shown on the right side of the gel. Values are expressed as the mean ± SD (n = 6). ^*P < 0.05 vs. control group, ^#P < 0.05 vs. D-gal alone-treated mice. ^@P < 0.05 young vs. old mice.