Research Paper Volume 16, Issue 7 pp 5887—5904
Cordyceps sinensis extract protects against acute kidney injury by inhibiting perforin expression in NK cells via the STING/IRF3 pathway
Figure 6. 2’-deoxyadenosine inhibited the activity of NK cells by regulating the STING/IRF3 signaling pathway in vitro. (A) Related target of 2’-deoxyadenosine with PTGS1 (COX-1), PTGS2 (COX-2), and MTAP based on DrugBank and TCMSP analysis. (B) Bioinformatics of PTGS1 (COX-1), PTGS2 (COX-2), STAT3 and its regulated STING/IRF3 from STRING PPI database. (C–H) The protein levels of COX-2, STAT3, STING, IRF3, and perforin in NK cells were evaluated by western blot analysis. (NK: naive NK cells. NK+IL-2: NK cells+IL-2 group. NK+IL-2+deo: NK cells+IL-2+2’-deoxyadenosine group. NK+deo: naive NK cells+2’-deoxyadenosine group). (I–K) The mRNAs levels of IFN-γ, TNF-α and CCL2 expression in NK cells were evaluated by qRT-PCR analysis. (NK: naive NK cells. NK+IL-2: NK cells+IL-2 group. NK+IL-2+deo: NK cells+IL-2+2’-deoxyadenosine group. NK+deo: naive NK cells+2’-deoxyadenosine group.) *P indicates a significant difference compared with the NK group. #P represents the difference compared with the NK+IL-2 group. P < 0.05. Values are shown as means ± SD from three independent experiments.