Research Paper Volume 16, Issue 7 pp 5887—5904

Figure 5. 2’-deoxyadenosine protected against AKI by inhibiting perforin expression in NK cells by regulating STING/IRF3 signaling pathway in vivo. (A) Immunofluorescence studies of perforin expression on NK cells after FA treatment. DAPI staining was used to determine the number of nuclei. Anti-perforin staining was used to determine perforin expression (×400). (B, C) Protein levels of perforin in the kidney were evaluated by western blot analysis. (D) The mRNA levels of perforin in the kidney were evaluated by qRT-PCR analysis. (E) Bioinformatics of STING (TMEM173) and its regulated IRF3 from STRING PPI database. (F, G) The mRNA levels of STING and IFN-γ in kidney were evaluated by qRT-PCR analysis. (H–J) Protein levels of STING, p-IRF3/IRF3 expression in kidney were evaluated by western blot analysis. ^*P indicates a significant difference between the NC group and the FA group. ^#P represents the difference between the FA+Deo group and the FA group. P < 0.05. Values are shown as means ± SD from three independent experiments. Representative images are shown from a total of six animals per group.