Research Paper Volume 16, Issue 6 pp 5651—5675
Single cell RNA-seq identifies a FOS/JUN-related monocyte signature associated with clinical response of heart failure patients with mesenchymal stem cell therapy
Figure 2. Subcluster analysis of the CD14+ monocytes. (A) Identification of highly-conserved DEGs/markers for CD14+ monocytes in the four groups, respectively. Three approaches for exploring DEGs were adopted. DEGs: differentially expressed genes. (B) NMF analysis for the reduction analysis of CD14+ monocytes based on all highly-conserved DEGs/markers. NMF: Nonnegative Matrix Factorization. (C) Distribution of all CD14+ monocytes from four groups. (D) Cell proportion of CD14+ monocytes was visualized, comprising 11 subclusters. (E) Cell distribution of all 11 identified subclusters. (F) Cell distribution of all 11 identified subclusters in the four groups. The CD14+ monocytes from response and non-response patients were quite different. (G) GSEA pipeline for showing the enriched terms for CD14+ monocytes from the “before response” group.