Research Paper Volume 16, Issue 6 pp 5634—5650
Identification and validation of ferroptosis-related gene SLC2A1 as a novel prognostic biomarker in AKI
Figure 10. SLC2A1 promotes HK-2 cell apoptosis and ferroptosis via ACSL4. (A, B) The protein level of ACSL4 and SLC2A1 in HR induced AKI mode when overexpressed SLC2A1, overexpressed SLC2A1+T, or T was verified by Western blot and relative quantification of these proteins; (C) The lipid ROS level was verified in HR induced AKI mode when overexpressed SLC2A1, overexpressed SLC2A1+T, or T; (D) The MDA level was verified in HR induced AKI model when overexpressed SLC2A1, overexpressed SLC2A1+T, or T; (E–G) The apoptosis rate was verified in HR induced AKI model when overexpressed SLC2A1, overexpressed SLC2A1+T, or T. *p < 0.05, **p < 0.01, ***p < 0.001. Abbreviations: T: Troglitazone; MDA: Malondialdehyde; ROS: Reactive Oxygen Species.