Research Paper Volume 16, Issue 6 pp 5601—5617
WTAP regulates the production of reactive oxygen species, promotes malignant progression, and is closely related to the tumor microenvironment in glioblastoma
Figure 7. Function enrichment analysis of WTAP. (A) The volcano plot showed differentially expressed genes (DEGs) based on the median value of WTAP expression in the TCGA cohort. (B) The heat map showed the relative expression levels of 1180 DEGs between WTAP high and low expression groups. (C) Enrichment analysis for GO term of up-regulated genes. (D) Enrichment analysis for GO term of down-regulated genes. (E) Enrichment analysis for KEGG pathway of up-regulated genes. (F) Enrichment analysis for KEGG pathway of down-regulated genes. (G) The PPI network showed the proteins that interact most closely with WTAP. (H) According to GSEA, the high expression of WTAP in the PI3K/AKT/mTOR signaling pathway was enhanced from the TCGA GBM dataset. (I, J) WTAP participates in the regulation of P-PI3K and P-AKT expression in U251 (I) and U87 (J) cells. *P < 0.05; **P < 0.01; ***P < 0.001.