Research Paper Volume 16, Issue 6 pp 5526—5544
Cinobufotalin regulates the USP36/c-Myc axis to suppress malignant phenotypes of colon cancer cells in vitro and in vivo
Figure 5. CBF interacts with USP36 and inhibits its enzyme activity to promote the ubiquitination and degradation of c-Myc. (A) Molecular docking diagram of CBF with USP36. (B) The inhibition of CBF on USP36 activity was determined by Ub-AMC hydrolysis assay. (C) The protein expression of USP36 and c-Myc in HCT116 cells with different doses of CBF was detected by western blot. (D) In vitro ubiquitination assay for HCT116 cells with the transfection of USP36 OE combined with or without CBF treatment. (E) CHX half-life assay. *p < 0.05 and **p < 0.01; Abbreviation: ns: no significance.