Research Paper Volume 16, Issue 6 pp 5526—5544
Cinobufotalin regulates the USP36/c-Myc axis to suppress malignant phenotypes of colon cancer cells in vitro and in vivo
Figure 3. USP36 overexpression promotes the stemness, growth, Oxa-resistance, and metastasis of colon cancer. (A) The xenograft formation of 1,000, 10,000, and 100,000 HCT116 cells transfected with EV or USP36 OE. (B) Tumorsphere formation frequency of HCT116 cells was calculated by ELDA. (C) Tumor growth curve. (D) The tumor was harvested from each group on day 28. (E) Weight of the harvested tumor. (F) Representative of gross view (left) and the number of metastatic nodules (right) of the liver from the metastatic murine model. (G) H&E staining for the liver tissues from the metastatic murine model (Scale bar = 100 μm). **p < 0.01 and ***p < 0.005.