Research Paper Volume 16, Issue 6 pp 5501—5525
Structural insights into human EMC and its interaction with VDAC
Figure 1. Discovery and structural study of the VDAC1-bound EMC complex. (A) Left panel: size-exclusion chromatography profile of the purified human EMC complex. Right panel: a silver staining image of the SDS-PAGE gel corresponding to the peak fraction in the profile. (B) Representative reference-free 2D cryo-EM average of the EMC. (C) Mass spectrometry analysis of the purified human EMC. (D) Two orthogonal views of the cryo-EM density map of human EMC-VDAC. The EMC, VDAC, and detergent micelle are colored in cyan, gray, and light gray respectively. (E) Side view of the cryo-EM density map and the ribbon diagram of human VDAC1, colored in rainbow to show the arrangement of the 19 β-strains.