Research Paper Volume 16, Issue 5 pp 4920—4942
Establishment of an immunogenic cell death-related model for prognostic prediction and identification of therapeutic targets in endometrial carcinoma
Figure 10. Validation of the role of IFNA2 in vitro experiments. (A) A CCK-8 assay was used to detect the effect of IFNα2 on the proliferation of HEC-1-A and AN3CA cells; (B, C) After treated with IFNα2 (IFNα2-1 (2×104U/mL) and IFNα2-2 (8×104U/mL)), the cloning ability of HEC-1-A and AN3CA cell lines decreased significantly; (D, E) EdU staining showing the effect of IFNα2 on HEC-1-A and AN3CA cells; (F) CCK-8 assay. After treated with IFNα2, the activity of HEC-1-A and AN3CA cell lines decreased significantly; (G, H) Transwell assay. After treated with IFNα2, the migration and invasion abilities of HEC-1-A and AN3CA cell lines were significantly decreased. *P<0.05, **P< 0.01, ***P<0.001.