Research Paper Volume 16, Issue 5 pp 4904—4919

MicroRNA-18a prevents senescence of mesenchymal stem cells by targeting CTDSPL

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Figure 5. miR-18a-5p overexpression reduced ROS levels of late passaged UCMSCs (passage 11). (A, B) Total ROS, mitochondrial mass and mitochondrial ROS levels of early and late passaged UCMSCs were detected by flow cytometry (A) and confocal microscope (B). (C) Flow cytometry analysis of the total ROS, mitochondrial mass and mitochondrial ROS levels of miR-18a-5p and control lentivirus vector transduced UCMSCs and (D) relative fluorescence intensity of miR-18a-5p overexpressing groups relative to control groups were quantified (n = 3). *p < 0.05; **p < 0.01.