Research Paper Volume 16, Issue 5 pp 4904—4919

Figure 3. Over-expression of CTDSPL induced premature senescence of UCMSCs. (A) SA-β-gal staining of control and CTDSPL over-expressed UCMSCs. (B) SA-β-gal positive cells were quantified (n = 3). (C) Morphology changes were quantified by flow cytometry. (D) Representative confocal microscopy images of GFP tagged control or CTDSPL transfected UCMSCs. F-actin was labeled with Alexa Fluor 633 conjugated phalloidin; nuclei were stained with Hoechst 33342. (E) Western blot assay of p16 expression after transfected with control or CTDSPL plasmid. (F) Western blot assay of RB and pRB expression after transfected with control or CTDSPL plasmid. ^***p < 0.001.