Research Paper Volume 16, Issue 6 pp 5184—5206
BMSCs-derived exosomes inhibit macrophage/microglia pyroptosis by increasing autophagy through the miR-21a-5p/PELI1 axis in spinal cord injury
Figure 1. BMSCs-derived exosomes inhibit BV2 microglia pyroptosis in vitro. (A) Morphology of exosomes under TEM; (B) NTA analysis of exosome size; (C) Western blot analysis of exosome surface markers; (D) The red fluorescent dye Dil-labeled exosomes was uptaken into macrophage/microglia; (E) Representative immunostaining image of IBA1 and GSDMD in the macrophage/microglia; (F) Quantification of fluorescence intensity; (G) Representative immunostaining image of IBA1 and Caspase-1 in the macrophage/microglia; (H) Quantification of fluorescence intensity (*p < 0.05, **p < 0.01,***p < 0.001).