Research Paper Volume 16, Issue 5 pp 4811—4831

Figure 3. PBK is identified as a novel target of FHND004 and FHND008. (A) The protein chip scan. The red arrow pointed to the positive control, the blue arrow pointed to the negative control, yellow arrow indicated the PBK protein. (B) Top 14 potential FHND008-associated proteins, including PBK. SNR: signal-to-noise ratio. (C) Biological processes of PBK protein based on the Gene ontology annotation, PBK is related to the negative regulation of MAPK. (D) The mRNA levels of PBK were significantly increased in MM samples. The signal level of PBK was shown on the y-axis. Patients designated as healthy donors with normal bone marrow plasma cells (NP, n = 22), monoclonal gammopathy of undetermined significance (MGUS, n = 44), or multiple myeloma (MM, n = 351) were sorted on the x-axis. (E, F) Kaplan-Meier analysis revealed the association of PBK expression with overall survival (OS) in TT2 (E) and HOVON65 (F) cohorts by log-rank test. Events/N means events of death/total patients. The data were expressed as the mean ± SD; p < 0.05 (*), p < 0.01 (* *) and p < 0.001 (* * *).