Research Paper Volume 16, Issue 5 pp 4759—4777
Network pharmacology and transcriptomic profiling elucidate the therapeutic effects of Ranunculus ternatus Thunb on liver fibrosis via MK3-NF-κB inhibition
Figure 5. β-sitosterol alleviates liver fibrosis by promoting LX-2 apoptosis. (A) COL1A1 was reduced after treating LX-2 with different doses of β-sitosterol for 48 h, showing concentration dependence. (B) Cellular activity was measured at different time points after treatment of LX-2 cells with different doses of β-sitosterol. (C) Cells treated with different doses of β-sitosterol were assayed for CASP8, cleaved-CASP8, CASP3, cleaved-CASP3 as well as BCL2 by WB. (D) After treatment of LX-2 cells with β-sitosterol, staining for TUNEL (green) and 4′,6-diamidino-2-phenylindole (DAPI) scale bar, 100 μm.