Research Paper Volume 16, Issue 5 pp 4670—4683
Echinatin mitigates sevoflurane-induced neurotoxicity through regulation of ferroptosis and iron homeostasis
Figure 2. Echinatin restrains sevoflurane-induced oxidative stress and inflammatory cytokines. HT22 cells were subjected to Echinatin treatment (0-40 μM) for a duration of 24 hours, followed by exposure to sevoflurane or control conditions. (A) Protein levels of GCL, NQO1, and Prx1 were measured by western blot. (B–D) The levels of MDA, GSH, TNF-α and IL-1β were measured by commercial kits. The data are presented as the mean ± SD. Ech, Echinatin; Sevo, sevoflurane. Compared with the Sevo+ Ech (0 μM) group, **P<0.01, ***P<0.001.