Research Paper Volume 16, Issue 5 pp 4670—4683
Echinatin mitigates sevoflurane-induced neurotoxicity through regulation of ferroptosis and iron homeostasis
Figure 1. Echinatin mitigates sevoflurane-induced apoptosis in HT22 cells. HT22 cells were subjected to Echinatin treatment (0-40 μM) for a duration of 24 hours, followed by exposure to sevoflurane or control conditions. (A) Cell vitality was measured by MTT assay. (B) Cell apoptosis was measured by flow cytometry. (C) LDH release was measured by LDH assay kits. (D) Protein levels of cleaved-caspase3, Bcl-2, and Bax were measured by western blot. The data are presented as the mean ± SD. Ech, Echinatin; Sevo, sevoflurane. Compared with the Sevo+ Ech (0 μM) group, **P<0.01, ***P<0.001.