Research Paper Volume 16, Issue 5 pp 4609—4630

Figure 3. MPC-EVs and MPC-Exo¹⁴⁰⁻ internalized by SCs induced Pax7 and MyoD significantly increased and promoted satellite cell proliferation. Activation of muscle satellite cells by MPC-EVs and MPC-Exo¹⁴⁰⁻. The process of SCs taking up myogenic extracellular vesicles was displayed under a live-cell confocal microscopy workstation, with (A) SC without MPC-Exond (B) treated with MPC-Exo^140- green fluorescence indicates uptake myogenic extracellular vesicles (Scale bar in 40 μm). (C) shows CCK8 results indicating a significant increase in cell proliferation after coincubation of SCs with MPC-EVs, especially in the MPC-Exo¹⁴⁰⁻ group. (D, E) present the results of cell cycle analysis by flow cytometry, revealing a significant increase in the number of cells in the S phase in the MPC-Exo¹⁴⁰⁻ group, while a significant decrease is seen in the MPC-Exo¹⁴⁰⁺ group. (F, G) show WB and RT-PCR results (fold change), showing that the expression of Pax7 and MyoD significantly increased in the MPC-Exo¹⁴⁰⁻ group, while they were significantly inhibited in the MPC-Exo¹⁴⁰⁺ group. Data: by one-way ANOVA analysis. ^nsp > 0.05, ^*p < 0.05, ^**p < 0.01 and ^***p < 0.001 vs. SC.