Research Paper Volume 16, Issue 5 pp 4609—4630

Figure 1. Significant downregulation of miR-140-5p expression following injury, contrasting with the expression trend of Pax7. Male wildtype C57BL/6 mice established damage model by intramuscular injection of 1.2% BaCl₂ into the gastrocnemius muscle. (A) displays the muscle repair and regeneration process post-acute damage to the gastrocnemius muscle caused by BaCl₂, as shown through histological examination staining (Scale bar in 250 μm). (B–D) present the RT-PCR results (fold change) illustrating the expression trends of miR-140-5p and Pax7 in the gastrocnemius muscle and circulation at different time points postinjury (n = 4 biological replicates). (E, F) demonstrate the binding pattern between miR-140-5p and Pax7, along with the dual luciferase assay results (n = 4), where WT refers to the wild type and MUT to the mutant type. The results are reported in the line/bar graph as mean ± SE; n = 4/group; different letters between bars mean P ≤ 0.05 analyses followed by non-paired Student’s t-test. ^*P < 0.05; ^**P < 0.01; ^***P < 0.001 vs. CON Group.