Research Paper Volume 16, Issue 5 pp 4095—4115

Figure 6. FoxO6 regulates hepatic lipid accumulation in FoxO6-KO mice. Mice were fed a high-fat diet at 4 weeks of age for 12 weeks (n = 6). (A) Western blotting was performed to examine the protein levels of FoxO6, ApoC3, and ApoB in the liver of FoxO6-KO mice. TFIIB was the loading control of the nuclear fractions, whereas β-actin was the loading control of the cytosolic fractions. Results are representative of three independent experiments. Bars in densitometry data represent the mean ± S.E, and significance was determined using one-factor ANOVA: ^**p < 0.01 vs. NTg. (B) Hepatic TG levels in FoxO6-KO mice. One representative result of three experiments yielding similar outcomes for each protein is shown. Results of one-factor ANOVA: ^*p < 0.05 vs. WT littermates. (C) Real-time PCR analyses were performed to measure the mRNA levels of FoxO6, MTP, ApoC3, ApoB, and ApoA1. Three independent experiments were performed and similar results were obtained. Results of one-factor ANOVA: ^*p < 0.05, ^**p < 0.01, ^***p < 0.001 vs. WT littermates. (D) Real-time PCR analyses were performed to measure the mRNA levels of PPARγ, ACC, and FAS. Results of one-factor ANOVA: ^*p < 0.05 vs. WT littermates. (E) Real-time PCR analyses were performed for measuring the mRNA levels of PEPCK, and G6Pase. Result of one-factor ANOVA: ^**p < 0.01 vs. WT littermates.