Research Paper Volume 16, Issue 5 pp 4095—4115

Figure 5. Deficiency of ApoC3 suppressed FoxO6-mediated lipid accumulation in AC2F liver cells. (A) AC2F cells were transiently transfected with ApoC3-siRNA (20 nM) for 24 h with or without FoxO6 (100 MOI). Cells were analyzed using western blotting using antibodies against FoxO6, ApoC3, and β-actin. Bars in the densitometry data represent the mean ± S.E., and significance was determined using one-factor ANOVA: ^#p < 0.05, ^##p < 0.01, ^###p < 0.001 vs. Normal; ^**p < 0.01 vs. FoxO6-CA. (B) Levels of ApoC3 were determined in the media of the cells. Three independent experiments were performed and similar results were obtained. Results of one-factor ANOVA: ^**p < 0.01 vs. non-treated cells; ^##p < 0.01 vs. FoxO6 virus-treated cells. (C) TG level of the media from FoxO6 with ApoC3-siRNA-treated cells. (D) Cellular TG concentration, after transfected cells were pre-incubated with ApoC3-siRNA (20 nM) for 24 h with or without FoxO6 (100 MOI), was measured using a colorimetric assay. Results of one-factor ANOVA: ^#p < 0.05, ^###p < 0.001 vs. non-treated cells; ^*p < 0.05, ^***p < 0.001 vs. FoxO6 virus-treated cells.